Laboratory of protein technologies
Artur Albert Hambardzumyan, PhD, head of lab.
Anichka Serezha Hovsepyan, PhD, leading researcher
Haykanush Onik Koloyan, PhD, senior researcher
Karine Georgievna Dyukova, PhD, senior researcher
Ani Misha Paloyan, PhD, senior researcher
Sona Voskan Avetisyan, PhD, senior researcher
Marina Hamlet Paronyan, PhD, researcher
Anna Vardges Mkhitaryan, PhD, junior researcher
Marieta Sergey Izmailyan, junior researcher
Narine Edvin Mikaelyan, junior researcher
Hasmik Suren Epremyan, PhD, engineer
The laboratory was established in 2010 as a Molecular Biology Laboratory, based on the molecular biology sector and a part of the former Genetic Engineering Laboratory. In 2017 renamed the Protein Technologies Lab. In 2018 the part of the previous laboratory of enzyme technology was merged with the laboratory. Currently, the laboratory has 10 employees.
Expression cloning of protein (enzymes) genes; isolation, purification and characterization of recombinant and natural proteins (enzymes); asymmetric enzymatic synthesis of amino acids; microbial synthesis of amino acids; bacterial melanin synthesis; obtaining of bacterial insecticides.
- Molecular biology
Genetic engineering; obtaining of cloneable amplicons; gene expression; DNA, RNA, protein, and other molecules (melanin) isolation, purification and characterization.
Determination of physicochemical and catalytic characteristics of enzymes; research and statistical analysis of mechanisms of enzyme catalysis.
Creating technologies based on strain-producers; creating technologies based on enzymes and their immobilized forms.
Search for genes based on genome analysis; the design of primers; construction of genes based on sequence data and uploading in the international databases; investigation of ligand-macromolecular interactions using docking analysis.
Identification of new bacteria; classification of bacteria on the basis of 16S rRNA gene sequences.
Installation and characterization of ionic channels in artificial two-layer lipid membranes; getting three-dimensional structures of proteins by homology modeling and applying the results in molecular docking experiments.
It is anticipated the obtaining of BAS and their strain-producers using genetico-selectional, gene engineering, biochemical engineering and immunological methods, as well as their study using biochemical and immunological methods.
In particular, it is planned to create the following classes of objects:
Enzymes and proteins (natural and recombinant) of high degree of purity for analytical and applied purposes.
- Various immobilized enzymes and biocatalysts with improved characteristics (activity, stability, possibility of demonstrating the activity in waterless or low-water content media, etc.) for using in different biotransformation processes.
- Monoclonal and Polyclonal Antibodies.
- Vaccines of various generations (protein forms and different expressible recombinant DNA constructs carrying the genes of epitopes of anti-genes).
- Recombinant strains producing amino acids, peptides, proteins and vaccines.
- Yerevan State University
- Yerevan State Engineering University (Polytechnic), Faculty of Chemistry
- Armenian State Agrarian University
- STC of Organic and Pharmaceutical Chemistry of NAS RA
- Institute of Physiology after L. Orbely of NAS RA
- Institute of Biochemistry after Hr. Buniatyan of NAS RA
- The Institute for Molecular Medicine, USA - Dr. Ghochikyan A.V.
- l'Université de Nantes, France - Prof. Sakanyan V.A.
- Quark Pharmaceuticals, Israel - Dr. Mett I.L.
- Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico - Dr. Petrosyan P.K.
- “Evonik Degussa GmbH” (Germany, France) – Dr. Karau A. and Dr. Versek S.
- SPC "Armbiotechnology" NAS RA with all laboratories.
- ISTC # A 1868, Development of a new technology for environmentally friendly bio-fertilizer complex based on nitrogen-fixing microorganisms and modified zeolites. H. H. Sargsyan,
Ph.D., leading researcher, 2010-2012.
- ISTC # A 1866, Natural Biological Substances (peptides) with Bactericidal properties toward various pathogens. F.N. Tkhruni, Ph.D., leading researcher, 2010-2012.
- ISTC # A 1701, Proline-rich polypeptide-1 and GX-NH2: molecular and genetic mechanisms of hematopoietic regulation. A.A. Galoyan, Dr.Sci., academician, 2009-2010.
- ISTC # A-1677, The Synthesis of Potential Biologically Active Heterocycle Substituted a-Amino Acids and Peptides. Investigation of their Antibacterial, Antiviral and Antitumor Properties. A.V. Geolchanyan, Ph.D., leading researcher, 2009-2011.
- ISTC # A-1247, Synthesis and Screening of Non-Protein Amino Acids and Peptides as Potential Constituents of Radiomodifying and Pharmacological Agents. A.V. Geolchanyan, Ph.D., leading researcher, 2005-2008.
- ISTC # A-683, Investigation of a new type of water soluble natural biologically active melanin: development of the technology for biosynthesis: extraction and purification; study of the chemical structure, physiological activity. A.S. Saghiyan, Dr.Sci., academician, 2002-2005.
- CRDF # AB2-2301-YE-02, Synthesis and study of new acetylcholine esterase and butyrilcholine esterase inhibitors. Gh.P. Halebyan, Dr.Sci., Profesor, 2001-2002.
- ANSEF 05-NS-biotech-0831-446, Energy Saving and Ecologically Acceptable Technology of L-Alanine Production. Gh. Halebyan, 2006.
- ANSEF № 2077-NS-biotech, Investigation of physiological activity of water-soluble bacterial melanin. А. Hovsepyan, 2010.
- ANSEF No PS-chemorg-2339, Synthesis of new neuro- protective drugs based on substituted small carbocycles, preliminary estimation of physiological activities by docking studies and electrochemical measurements. A. Mikaelyan, 2011.
- ANSEF № 3560, Bacillus thuringiensis strains melanin as stimulant of plant growth in agriculture. S. Avetisyan, 2014:
- ANSЕF biоtеch-4241, Bacterial Melanin as a potential means of treating Parkinson's disease pre-clinical research models of neurodegeneration. A. Hovsepyan, 2016.
- ANSEF Plant- 4720, New highly effective insecticides based on melanogen strains of Bacillus thuringiensis. S. Avetisyan, 2017.
- RA Thematic 13-2I344, Construction of recombinant strains with high hydantoinase activity, hydantoinase isolation and characterization. A.S. Hovsepyan, Ph.D., leading researcher, 2013-2015.
- RA Thematic 13-2I359, Cloning of genes of alfa-amylase and glucoamylase of technological value and characterization of corresponding enzymes. A.A. Hambardzumyan, Ph.D., leading researcher, 2013-2015.
- RA Thematic 11-2i381, Obtaining the strains carrying recombinant plasmids coding inducible D- and L-aminoacylases and characterization of corresponding enzymes.
A.A. Hambardzumyan, Ph.D., leading researcher, 2011-2013.
- RA Thematic 462, Preparation of immobilized preparations of D-amino acid acylase, hydantoinase and carbamoylase and applying in biotransformation processes.
A.A. Hambardzumyan, Ph.D., leading researcher, 2008-2010.
- RA Thematic 2005-252, Preparation and study of biologically active compounds (amino acids and their derivatives, peptides and amino ethers) by biotransformation process.
Gh.P. Halebyan, Dr.S., leading researcher, 2005-2007.
- RA Thematic 2000-265, Obtaining of high-producing strains of α-amylase and the creation of technologies for the production of these enzymes on the basis of these strains.
A.A. Hambardzumyan, Ph.D., leading researcher, 2000-2001.
- RA Thematic 92-585, Development of technology for the isolation and the purification of hexokinase, glucose-6-phosphate dehydrogenase, glucose oxidase and peroxidase to be used for components in diagnostic kits for glucose determination in serum. A.A. Hambardzumyan, Ph.D., leading researcher, 1993-1995.
- RA MES SCS - BRFFR-2014 13RB-063, Promoting plant growth and development using Bacillus thuringiensis K1 insecticidal strains synthesized bacterial melanin. A. Hovsepyan, 2014.
- RA MES SCS - BRFFR-2016 RB16-53, Acquisition and use of biopreparations based on Bacillus thuringiensis for plant growth promotion and pest management. A. Hovsepyan, 2017.
- 16GE-034, Use of hypertrophilic carbamoylase N-carbamoyl-D-amino acids in enzymatic hydrolysis. A. Hovsepian, 2017.
Young researchers’ grants
- 16YR-2I068, Directed modification of recombinant D-hydantoinase of Geobacillus stearothermophilus for improvement of its catalytic properties, Aganyants Hovsep, 2016.
- 16YR-2I016, Obtaining strain-producer of ɑ-amylase on the basis of ɑ-amylase gene of Bacillus amyloliquifaciens and characterization of the recombinant enzyme. A. Paloyan, 2016.
- Obtaining of bacterial water-soluble melanin with phytostimulating activity.
- Aghajanyan A.E., Hambardzumyan A.A., Hovsepyan A.S., Asaturian R.A., Vardanyan A.A. and Saghiyan A.A. Isolation, purification and physicochemical characterization of water-soluble Bacillus thuringiensis melanin. Pigment Cell Res. 2005, 18; 130–135.
- Paloyan A.M., Hambardzumyan A.A. and Halebyan Gh.P. Isolation, purification, and characterization of phenylpyruvate transaminating enzymes of Erwinia carotovora Biochemistry (Moscow), 2012, 77(1): 98-104.
- Petrosyan T.R., Gevorkyan O.V., Meliksetyan I.B., Hovsepyan A.S., Manvelyan L.R. Neuroprotective action of bacterial melanin in rats after corticospinal tract lesions Pathophysiology, 2012, 19, 71-80.
- Paloyan A.M., Stepanyan L.A., Dadayan S.A., Hambardzumyan A.A., Halebyan Gh.P. and Saghiyan A.S.Catalytic properties of enzymes from Erwinia carotovora involved in transamination of phenylpyruvate Applied Biochemistry and Microbiology, 2013, 49(2): 106–112.
- Petrosyan T.R., Gevorkyan O.V., Chavushyan V.A., Meliksetyan I.B., Hovsepyan A.S., Manvelyan L.R. Effects of bacterial melanin on motor recovery and regeneration after unilateral destruction of substantia nigra pars compacta in rats Neuropeptides, Elsevier, 2014, 48, 37-46.
- Petrosyan T.R., Hovsepyan A.S. Bacterial melanin improves cognitive impairment induced by cerebral hypoperfusion in rats Journal of Motor Behavior, 2014, 46(6), 469-475.
- Sakanyan V., Hulin P., Alves de Sousa R., Silva V. A. O., Hambardzumyan A., Nedellec S., Tomasoni C., Logé C., Pineau Ch., Roussakis C., Fleury F. & Artaud I. Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1 Scientific Reports (Nature Publishing Group), ISSN 2045-2322 (online), 17 February 2016, 6, 21088, doi: 10.1038/srep21088, p. 1-14.
- Hambardzumyan A.A., Mkhitaryan A.V., Paloyan A.M., Dadayan S.A., and Saghyan A.S. Catalytic Properties of Aminoacylase of Strain Rhodococcus Armeniensis AM6.1 Applied Biochemistry and Microbiology, 2016, 52(3): 250–255.
- Petrosyan T.R., Ter-Markosyan A.S., Hovsepian A.S. Detection of Ca2+-dependent acid phosphatase activity identifies neuronal integrity in damaged rat central nervous system after application of bacterial melanin Neural Regen Res, 2016, 11(7), 1147-1152.
- Hambardzumyan A.A., Mkhitaryan A.V., Paloyan A.M., and Dadayan S.A. Covalent Immobilization of D-Aminoacylase of Strain Rhodococcus armeniensis AM6.1 and the Characteristics of the Biocatalyst, Applied Biochemistry and Microbiology, 2017, 53(1); 20-24.