SCIENTIFIC AND PRODUCTION CENTER “ARMBIOTECHNOLOGY” NAS RA

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Research Units

Research Units

 

Artur Hambardzumyan

 
Head of Laboratory
PhD in Biology
e-mail: arthambardzumyan@armbiotech.am 

 

Laboratory of Protein Technologies

 

Laboratory of Protein Technologies was founded in 2010.

 

Field of Activity

  • Molecular biology:

gene engineering, obtaining of cloning amplicons, gene expression, DNA, RNA, isolation, purification and characterization of protein and other molecules (melanin)

  • Biochemistry:

determination of physicochemical and catalytic properties of enzymes, study of mechanisms of enzymatic catalysis and statistical analysis

  • Biotechnology:

development of technologies based on strain-producers, development of technologies based on enzymes and their immobilized forms

  • Bioinformatics:

search for genes based on genome analysis, design of primers, construction of genes based on sequence data and placement in international databases, investigation of ligand-macromolecular interaction by docking analysis

  • Microbiology:

detection of new bacteria, classification of bacteria based on 16S rRNA sequencing.

  • Biophysics:

reconstruction and characterization of ionic channels in artificial bilayer lipid membranes, obtaining of a three-dimensional protein structure from homologous modeling and its application in molecular docking experiments

 

RESEARCH MAIN DIRECTIONS

  • Expression cloning of protein (enzyme) genes
  • Isolation, purification and characterization of recombinant and natural proteins (enzymes) asymmetric enzymatic synthesis of amino acids
  • Microbiological synthesis of amino acids
  • Bacterial melanin synthesis
  • Obtaining of bacterial insecticidal preparations    

 

MAIN INVESTIGATION RESULTS

  • Obtaining of recombinant strain-producers of L-arginine (E. coli - up to 35 g / l and Br. Flavum - up to 31 g / l) and study of physiological and biochemical properties of these strain-producers
  • Obtaining of strain-producers of bacterial, water-soluble, biologically active melanin (particularly plant growth stimulators)
  • Obtaining of strain-producers of L- and D-aminoacylases
  • Isolation and characterization of a number of bacterial amino acid transaminases (alanine, aspartate, aromatic)
  • Clarification of a transport nature of overproduction of certain amino acid strain-producers (L-alanine, L-proline)
  • Development of technologies for production of a number of enzymes: glucose oxidase, peroxidase, glutamate dehydrogenase, etc.
  • Characterization of potential ligands for a number of target proteins (EGFR, MAPK14, TLR4, etc.) by molecular docking    

 

MAIN OBJECTIVES AND TASKS

  • It is envisaged to obtain BAS and their strain-producers by genetic-selection, gene engineering, biochemical engineering and immunological methods and to study them by biochemical and immunological methods. In particular, it is envisaged to create the objects of the following classes:- high purity enzymes and proteins (natural and recombinant) for analytical and practical purposes- various immobilized enzymes and biocatalysts with improved characteristics (activity, stability, possible activity in anhydrous or low water mixtures, etc.) for using in different types of biotransformation processes- monoclonal and polyclonal antibodies- vaccines of different generations (protein constructs and constructs of various recombinant DNAs carrying antigen epitope genes)- recombinant strains producing amino acids, peptides, proteins (enzymes) and vaccines    

 

LIST OF GRANT PROJECTS

  1. 20APP-4C018, Obtaining and use of insecticidal preparations and plant growth stimulants based on strains of Bacillus thuringiensis, 24 million AMD, Head of Theme: Avetisyan Sona, 2020-2022.
  2. SCS MES RA-BRFBR 18BL-040 “Use of bacterial melanin as a stimulant for growth and development of rare pharmaceutical and local high ornamental plants”, 2018-2020 (Project Manager: A. Hovsepyan, PhD).
  3. ISTC #A‑2289 “Synthesis and screening of a new generation of optically active non-proteinogenic  α-amino acids, peptides and polymers containing unsaturated groups in the side chain”, 2017-2020 (Project Manager: Academician of NAS RA, Prof. A. Saghyan).
  4. 16GE-034 “Application of of hyperthermophil carbamoylase in enzymatic hydrolysis of N-carbamoyl-D-amino acids”, 2017-2019 (Project Manager: A. Hovsepyan, PhD).
  5. ANSEF  Plant-4720 “New high-efficiency insecticidal preparations based on the melaninogenic strains of Bacillus thuringiensis”, 2017 (Project Manager: S. Avetisyan, PhD).
  6. SCS MES RA-BRFBR-2016 ՀԲ16-53 “Preparation and application of biopreparations based on the Bacillus thuringiensis for promoting plant growth and protection against pests”, 2016-2018 (Project Manager: A. Hovsepyan, PhD).
  7. 16YR-2I068 “Targeted modification of recombinant D-hydroxinase of Geobacillus stearothermophilus to improve the catalytic properties of the enzyme”, 2016-2018 (Project Manager: H. Aganyants, PhD).
  8. 16YR-2I016 “Obtaining of the strain-producer of  α-amylase on the base of the gene of Bacillus amyloliquefaciens α-amylase and recombinant enzyme characterization”, 2016-2018 (Project Manager: A. Paloyan, PhD).
  9. ANSЕF biоtеch-4241 “Microbial melanin as a means of preclinical study of potential treatment for Parkinson’s disease using neurodegeneration models”, 2016 (Project Manager: A. Hovsepyan, PhD).
  10. SCS MES RA-BRFBR-2014 13РБ-063 “Stimulation of plant growth and development by the use of bacterial melanin synthesized by the insecticidal strain of Bacillus thuringiensis K1”, 2014-2016 (Project Manager: A. Hovsepyan, PhD).
  11. ANSEF No. 3560 “Bacillus thuringiensis strain melanin as a stimulant for plant growth in agricultura”, 2014 (Project Manager: S. Avetisyan, PhD).
  12. 13-2I344 “Construction of recombinant strains possessed by high activity of hydantoinase, isolation and characterization of hydantoinase”, 2013-2015 (Project Manager: A. Hovsepyan, PhD).
  13. 13-2I359 “Cloning of technologically valuable α-amylase and glucoamylase genes and characterization of relevant enzymes”, 2013-2015 (Project Manager: A. Hambardzumyan, PhD).
  14. 11-2i381 “Derivation of strains carrying recombinant plasmids encoding inducible D- and L-aminoacylase and characterization of relevant enzymes”, 2011-2013 (Project Manager: A. Hambardzumyan, PhD).
  15.  ANSEF No. PS-chemorg-2339 “Synthesis of new neuroprotective drugs on the basis of small, substituted carbocycles, preliminary assessment of physiological activities by docking analysis and electrophysiological measurements”, 2011 (Project Manager: A. Mikaelyan).
  16. ISTC #A‑1868 “Development of a new technology for the production of environmentally friendly complex biofertilizers based on nitrogen-fixing bacteria and modified zeolites”,  2010-2012 (Project Manager: H. Sargsyan).
  17. ISTC #A‑1866 “Natural biological compounds (peptides) with bactericidal properties against various pathogens”, 2010-2012 (Project Manager: F. Tkhruni, PhD).
  18. ISTC # A-1677, Synthesis of -amino acids and peptides substituted in potentially bioactive heterocycles. Study of their antibacterial, antiviral and anti-tumor properties, 375 000 USD, PhD in Chemistry Geolchanyan A.V., 2009-2011.

 

KEY PUBLICATIONS

Scientific articles

  1. Дюкова К.Г., Амбарцумян А.А., Алебян Г.П. Ковалентная иммобилизация очищенной тирозин-фенол – лиазы из Citrobacter freundii на неорганических носителях. Биотехнология, 2011, №2, 58-65.
  2. Aghajanyan A.A., Asaturyan R.A., Hambardzumyan A.A., Sargsyan L.B., Hovsepyan A.S., Vardanyan A.H. and Saghyan A.S. Obtaining of water soluble microbial melanin and study of its some properties Applied Biochemistry And Microbiology, 2011, 47(5), 500–506, Original Russian Text  Aghajanyan A.A., Asaturyan R.A., Hambardzumyan A.A., Sargsyan L.B., Hovsepyan A.S., Vardanyan A.H., Saghyan A.S. 2011, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2011, 47(5): 551–557. DOI: 10.1134/S0003683811050024.
  3. Paloyan A.M., Hambardzumyan A.A. and Halebyan Gh.P. Isolation, purification, and characterization of phenylpyruvate transaminating enzymes of Erwinia carotovora Biochemistry (Moscow), 2012, 77(1): 98-104, Original Russian Text Paloyan A. M., Hambardzumyan A. A., Halebyan Gh. P. 2012, published in Biokhimiya, 2012, 77(1): 123-129. DOI: 10.1134/S0006297912010129
  4. Gevorgyan G.K., Davtyan M.A. and Hambardzumyan  A.A. Simple Purification Procedure of D-Amino-Acid Oxidase from Candida guilliermondii NP-4 Microbiology (Mikrobiologiya),  2012, 81(4): 498-504. DOI: 10.1134/S0026261712040078
  5. Mikaelyan A.R., Vardanyan A.S., Sargsyan V.A., Hambardzumyan A.A. Characterization of pyrethroid – insect ion channel interactions via cyclic voltammetric technique Biolog. Journal of Armenia, 2012, 64(3); 76-82.
  6. Yepremyan  H.S. Obtaining and properties  of intracellular aminoacylase Escherichia coli. Biochemistry and biotechnology research and development /Eds: Varfolomeev S.D., Zaikov G. E., Krylova L.P., 2012, New-York, Nova Science Publishers, chapter 5, p. 35-38.
  7. Аганянц О.А., Мхитарян А.В., Дадаян А.С., Колоян А.О., Овсепян А.С. Разработка метода получения L- и D-валина из D,L-валина с использованием L-аминоацилазы. Биолог. журн. Армении, 2012; 3(64):91–96. http://biology.asj-oa.am/10935/1/91.pdf.
  8. Gyulkhandanyan G.V., Ghazaryan R.K., Paronyan M.H., Ulikhanyan Gh.I., Gyulkhandanyan A.G., Sahakyan L.A. Antimicrobial activity of new porphyrins of synthetic and natural origin. Mechanisms for Low-Light Therapy VII, edited by M.R. Hamblin, J. Anders, J.D. Carroll, Proc. of SPIE, 2012; 8211:821107-1–821107-9. Doi: 10.1117/12.908634.
  9. Petrosyan T.R., Gevorkyan O.V., Meliksetyan I.B., Hovsepyan A.S., Manvelyan L.R. Neuroprotective action of bacterial melanin in rats after corticospinal tract lesions. Pathophysiology, 2012; 19(2):71–80. DOI:10.1016/j.pathophys.2011.12.003.
  10. Chil-Hakobyan L.A., Hambardzumyan A.A. and Chakhalyan A.Kh. Crystal formation peculiarities in pigmented cultures of Bacillus thuringiensis Applied Biochemistry and Microbiology, 2013, 49(1): 34–40. Original Russian Text Chil-Hakobyan L.A., Hambardzumyan A.A., Chakhalyan A.Kh., 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, 49(1): 41–47. DOI: 10.1134/S0003683813010031
  11. Paloyan A.M., Stepanyan L.A., Dadayan S.A., Hambardzumyan A.A., Halebyan Gh.P. and Saghiyan A.S.Catalytic properties of enzymes from Erwinia carotovora involved in transamination of phenylpyruvate Applied Biochemistry and Microbiology, 2013, 49(2): 106–112. Original Russian Text  Paloyan A.M., Stepanyan L.A., Dadayan S.A., Hambardzumyan A.A., Halebyan Gh.P., Saghiyan A.S., 2013, published in Prikladnaya Biokhimiya I Mikrobiologiya, 2013, 49(2): 129–135. DOI: 10.1134/S0003683813020129
  12. Melkonyan L.H., Avetisova G.Y., Hambardzumyan A.A., Chakhalyan A.Kh. and Saghyan A.S. Study of regulation of some key enzymes of L-alanine biosynthesis by Brevibacterium flavum producer strains Applied Biochemistry and Microbiology, 2013, 49( 2): 120–124. Original Russian Text Melkonyan L.H., Avetisova G.Y., Hambardzumyan A.A., Chakhalyan A.Kh., Saghyan, A.S. 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, 49(2): 144–148. DOI: 10.1134/S0003683813020129
  13. Aghajanyan A.E., Tsaturyan A.O., Hambardzumyan A.A. and Saghyan A.S. Obtaining the zwitterionic form of L-lysine from L-lysine monohydrochloride by electrodialysis Membrane Water Treatment, 2013, 4(1): 1-9. DOI: 10.12989/mwt.2013.4.1.001
  14. Mikaelyan A.R., Vardanyan A.S., Torosyan G.G., Grigoryan A.M., Hambardzumyan A.A. The study of structure-activity relationship of some pyrethroid-like compounds by means of docking analysis and physical measurements Proceedings of SEUA. Series “Chemical and environmental technologies”, 2013, 16(1): 49-56.
  15. Gyulkhandanyan A.G., Ghazaryan R.K., Gasparyan V.K., Sargsyan H.O., Madoyan R.A., Gyulkhandanyan A.G., Paronyan M.H., Stasheuski A.S., Knyukshto V.N., Dzhagarov B.M., Gyulkhandanyan G.V. Complexation of porphyrins with silver and zeolite nanoparticles. Nanotechnology VI, edited by R. Adelung, Proc. Of SPIE, 2013; 8766:87660H-1–87660H-8. Doi: 10.1117/12.2017142.
  16. Mkhitaryan A.V. Isolation and identification of strain with D‑aminoacylase activity: preliminary characterization of the enzyme Biological Journal of Armenia, 2013, № 4, pp. 58-63.
  17. Azaryan K.G., Popov Yu.G., Hovsepyan A.S., Avetisyan S.V. Perspectives of natural stimulators application in plant cultivation. Procceding of the Eurasian symposium on vegetables and greens, Acta Horticulturae, 2014; 1033:65–75.
  18. Gyulkhandanyan G.V., Ghazaryan R.K., Paronyan M.H., Gyulkhandanyan A.G., Sheyranyan M.A., Dzhagarov B.M., Tuchina E.S., Korchenova M.A., Tuchin V.V. Photophysical properties and photodynamic efficiency of cationic porphyrins. Dynamics and Fluctuations in Biomedical Photonics XI, edited by V.V. Tuchin, K.V. Larin, M.J. Leahy, R.K. Wang, Proc. of SPIE, 2014; 8942:894212-1–894212-9. Doi: 10.1117/12.2039715.
  19. Petrosyan T.R, Chavushyan V.A. Hovsepyan A.S. Bacterial melanin increases electrical activity of neurons in substantia nigra pars compacta. J. Neural. Transm., 2014; 121(3):259–265. DOI 10.1007/s00702-013-1095-9.
  20. Petrosyan T.R., Gevorkyan O.V., Chavushyan V.A., Meliksetyan I.B., Hovsepyan A.S., Manvelyan L.R. Effects of bacterial melanin on motor recovery and regeneration after unilateral destruction of substantia nigra pars compacta in rats. Neuropeptides, 2014; 48(1):37–46. DOI: 10.1016/j.npep.2013.10.001.
  21. Petrosyan T.R., Gevorkyan O.V., Hovsepyan A.S. Effects of bacterial melanin on movement, posture, and skilled balancing deficits after unilateral destruction of substantia nigra pars compacta in rats. J. Mot. Behav. 2014; 46(1):67–72. DOI: 10.1080/00222895.2013.865588.
  22. Petrosyan T.R., Hovsepyan A.S. Bacterial melanin crosses the blood–brain barrier in rat experimental model. Fluids Barriers CNS, 2014; 11:20. DOI: 10.1186/2045-8118-11-20.
  23. Petrosyan T.R., Hovsepyan A.S. Bacterial melanin improves cognitive impairment induced by cerebral hypoperfusion in rats. J. Mot. Behav., 2014: 46(6):469–475. DOI: 10.1080/00222895.2014.947912.
  24. Gevorkyan O.V., Meliksetyan I.B., Petrosyan T.R., Hovsepyan A.S., Bacterial melanin promotes recovery after sciatic nerve injury in rats. Neural Regen. Res., 2015; 10(1):124–127. DOI:10.4103/1673-5374.150719.
  25. Petrosyan T.R., Gevorgyan O.V. Hovsepyan A.S. and Ter-Markosyan A. S. Effects of bacterial melanin on neuronal activity in the rat sensorimotor cortex. Neurophysiology, 2015; 47(6):448–453. DOI: 10.1007/s11062-016-9554-1.
  26. Sakanyan V., Hulin P., Alves de Sousa R., Silva V. A. O., Hambardzumyan A., Nedellec S., Tomasoni C., Logé C., Pineau Ch., Roussakis C., Fleury F. & Artaud I. Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1 Scientific Reports (Nature Publishing Group), ISSN 2045-2322 (online), 17 February 2016, 6, 21088, doi: 10.1038/srep21088, p. 1-14.
  27. Petrosyan T.R., Ter-Markosyan A.S., Hovsepyan A.S. Detection of Ca2+-dependent acid phosphatase activity identifies neuronal integrity in damaged rat central nervous system after application of bacterial melanin. Neural Regen. Res., 2016;11(7):1147–1152 DOI:10.4103/1673-5374.187055
  28. Hambardzumyan A.A., Mkhitaryan A.V., Paloyan A.M., Dadayan S.A., and Saghyan A.S. Catalytic Properties of Aminoacylase of Strain Rhodococcus Armeniensis AM6.1 Applied Biochemistry and Microbiology, 2016, 52(3): 250–255. Original Russian Text Hambardzumyan A.A., Mkhitaryan A.V., Paloyan A.M., Dadayan S.A., Saghyan A.S., 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, 52(3): 272–278. DOI: 10.1134/S0003683816030029.
  29. Дюкова К.Г., Измаилян М.С., Манукян Л.С., Кочикян В.Т.. Выделение, очистка, характеризация инулиназы из клубней топинамбура (Helianthus tuberosus.) Биолог. жур. Армении, 2016, N 2 (68), с.30-37.
  30. Hambardzumyan A.A., Mkhitaryan A.V., Paloyan A.M., and Dadayan S.A. Covalent Immobilization of D-Aminoacylase of Strain Rhodococcus armeniensis AM6.1 and the Characteristics of the Biocatalyst, Applied Biochemistry and Microbiology, 2017, 53(1); 20–24. Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 1, pp. 26–30. DOI: 10.1134/S0003683817010057
  31. Hambardzumyan A.A. Statistical analyses of enzyme kinetics: simple Michaelis-Menten and bi-bi ping-pong. Biolog. Journal of Armenia, 2017, 69(2); 6-12.
  32. Aghajanyan A.E., Vardanyan A.A., Hovsepyan A.S., Hambardzumyan A.A., Filipenia V., Saghyan A.S. Development of technology for obtaining water-soluble bacterial melanin and determination of some of pigment properties. BioTechnologia: Journal of Biotechnology, Computational Biology and Bionanotechnology, 2017, 98(4) 315-322. doi.org/10.5114/bta.2017.72292.
  33. Hambardzumyan A.A. Statistical analyses of enzyme kinetics: inhibition. Biolog. Journal of Armenia, 2018, 70(1); 35-44.
  34. Iradyan M.A., Iradyan N.S., Hambardzumyan A.A., Panosyan H.A., Roussakis C. and Sakanyan V.A. Synthesis of furfuryl derivatives of 4-allyl-1-(4-hydroxy-3-nitrobenzyl)-3-[2-(4-alkoxyphenyl)quinolin-4-yl]-4,5-dihydro-1H-1,2,4-triazol-5-thions and their cytotoxic action on human cancer cells  Chemical Journal of Armenia 2018, 71(4), 559-570.
  35. Дюкова К. Г., Измаилян М.С.,  Киносян М.А., Давидян Т.С., Кочикян В.Т. Сравнитель­ный анализ оптимальных условий функционирования штаммов-продуцентов с выраженной аспартазной активностью. Биотехнология, 2018, Т. 34, N 1, с. 24-34.
  36. Petrosyan T.R., Hovsepyan A.S., Avetisyan S.V., Kurianc N. In-vitro Effects of Bacterial Melanin in Macrophage. Neuroimmune Biology, 2019, v.7, p. 199–206. - “RAW 264.7” Cell Culture.
  37. Iradyan M., Iradyan N., Hulin Ph., Hambardzumyan A., Gyulkhandanyan A., Alves de Sousa R., Hessani A., Roussakis Ch., Bollot G., Bauvais C. and Sakanyan V. Targeting Degradation of EGFR through the Allosteric Site Leads to Cancer Cell Detachment-Promoted Death Cancers 2019, 11, 1094; doi:10.3390/cancers11081094.
  38. Aветисян С. В., Паронян М. Г., Оганнесян С. С., Овсепян А. С. Меланиногенные штаммы Bacillus thuringiensis: перспективы их применения. “Доклады НАН РА”, 2020, 120(1):74-80.
  39. Koloyan H., Avetisyan S., Paronyan M., Aganyants H. , Hovsepyan A.  Obtaining new recombinant strains of Brevibacterium flavum containing heterologous Geobacillus stearothermophilus gene argJ. "Research Journal of Biotechnology", 2020, 15(5):71-85.
  40. Башилов А.В., Шутова А.Г., Овсепян А.С., Аветисян С.В. Использование биопрепарата на основе меланиногенного штамма B. thuringiensis  при подготовке посадочного материала для озеленения городской среды. “Вестник фонда фунгаментальных исследований" Минск, 2020, 3(93):48-56.
  41. Aganyants H., Weigel P., Hovhannisyan Ye., Lecocq M., Koloyan H., Hambardzumyan A., Hovsepyan A., Hallet  J.-N. and Sakanyan V. Rational Engineering of the Substrate Specificity of a Thermostable D-Hydantoinase (Dihydropyrimidinase). High-Throughput 2020, Volume 9, Issue 1, 5; doi:10.3390/ht9010005.
  42. Paloyan A.M., Melkonyan L.H., Sargsyan A.S., Mkhitaryan A.V., Dyukova K.G., Hambardzumyan A.A. Production and Properties of a Novel Raw Starch Hydrolyzing -Amylase from Bacillus amyloliquefaciens MDC1974.  Electronic Journal of Natural Sciences of NAS of Armenia  2020, 34 (1), 4-11.
  43. Дюкова К.Г., Измаилян М.С., Палоян А.М., Амбарцумян А.А. Улучшение каталитических свойств штамма-продуцента аспартазы Pectobacterium carotovorum в процессе получения L-аспарагиновой кислоты Биотехнология 2020, 36(1), 30-35.

 

Patents

  1. Paloyan A., Dyukova K., Papoyan A., Aghajanyan A., Hambardzumyan A., Halebyan Gh. // Method of obtaining L-phenylalanine RA Patent № 2479 A, Application № AM20100138, C12P 13/00, Publication Date: 25.01.2011.
  2. Vardanyan A,,   Aghajanyan A.,   Hovsepyan A.,   Saghyan A..   Microbiological method of obtaining water-soluble melanin, RA Patent № 3328 A, Application № AM20180018,  C12N 1/00, C12P 13/00,  Publication Date: 19.02.2019.
  3. Aghajanyan A., Vardanyan A., Hambardzumyan A. The method of melanin obtaining Patent of Armenia № 3397 A, Application № AM20180138, C07G 17/00, C12H 1/00, Publication Date: 01.07.2020, 6p.

Abstracts Presented at Conferences

  1. Yepremyan  H.S. Artsruni G.K. Intracellular aminoacylase of Escherichia coli. Abstr. of reports on 6th Moscow International Congress “Biotechnology: state of the art and prospects of development”, 2011, Moscow, March 21-25, p. 269-270.
  2. Ulikhanyan Gh.I., Gyulkhandanyan G.V., Paronyan M.H., Ghazaryan R.K., Sahakyan L.A. Antimicrobial activity of new cationic porphyrins of synthetic and natural origin. 2nd International Conference on Organic Chemistry: “Advances in Heterocyclic Chemistry” Tbilisi, Georgia, September 25–27, 2011, p. 199–200.
  3. Dyukova K. Halebyan Gh. Catalitic Properties of Enzymes Aspartase and Fumaraze of Erwinia Carotovora. Scientific seminar “Modern state of biotechnological developmemts and ways of commercialization”, Book of abstracts, Armenia, Yerevan, September 11-12, 2012, p.72-73.
  4. Hovsepyan A.S., Koloyan H.O., Avetisyan S.V., Hovhannisyan Y.A. Approaches for obtaining highly active strains-producers of arginine of Esherichia coli. Book of Abstracts of the ISTC Scientific Seminar “Modern state of biotechnological developments and ways of commercialization”, Yerevan, Armenia, September 11–12, 2012, p. 81. ISBN 978-99941-2-726-9.
  5. Hovsepyan A.S., Koloyan H.O., Avetisyan S.V., Hovhannisyan Y.A. Approaches for obtaining highly active strains-producers of arginine of Esherichia coli. Book of Abstracts of the ISTC Scientific Seminar “Modern state of biotechnological developments and ways of commercialization”, Yerevan, Armenia, September 11–12, 2012, p. 81. ISBN 978-99941-2-726-9.
  6. Paronyan M.H., Hovsepyan A.S., Ghazaryan R.K., Gyulkhandanyan A.G., Gyulkhandanyan G.V. Antibacterial activity of cationic porphyrins in vitro and in vivo. Book of Abstracts of the ISTC Scientific Seminar “Modern state of biotechnological developments and ways of commercialization”, Yerevan, Armenia, September 11–12, 2012, p. 85. ISBN 978-99941-2-726-9.
  7. Azaryan K.G., Popov Yu.G., Hovsepyan A.S., Avetisyan S.V. The stimulation of vegetables growth and development by treatment with some biopreparations. Eurasian symposium on vegetables and greens, Yerevan, Armenia, October 16–20, 2012, p. 22.
  8. Balayan A.M., Ghochikyan V.T., Manukyan L.S., Andreasyan N.A., Afyan K.B., Dyukova K.G.. Development of Technological Process for obtaining Ethyl Alcohol from Inulin-Containing Raw Material. “Contribution of the young generation in the development of biotechnology” 2 nd International Scientific Conference of Young Reserchers. October 1-4, 2013, Yerevan, p.13-14.
  9. Avetisyan S.V., Hovsepyan A.S., Aghajanyan A.E., Azaryan K.G., Petrosyan T.R., Saghyan A.S. Water-soluble bacterial melanin: obtaining, biological activity, application perspectives. 2nd International Scientific Conference of Young Researchers “Contribution of the young generation in the development of biotechnology” Dedicated to the 70th Anniversary of the National Academy of Sciences of Armenia, Yerevan, October 1–4, 2013, p. 48.
  10. Gaybakyan L.D., Hambardzumyan A.A. Alanine-2-Ketoglutarate Aminotransferase of Brevibacterium flavum: Isolation, Purification and Primary Characteristics. Abstract in book of articles and abstracts of 2nd International Scientific Conference of Young Researchers on Biotechnology, General and Applied Microbiology, Chemistry, Biochemistry, Molecular Biology and Genetics, Environmental Protection “Contribution of young generation in the development of biotechnology”, Dedicated to the 70th Anniversary of the National Academy of Sciences, October 1-4, 2013, Yerevan, Armenia, p. 129.
  11. Melkonyan L.H., Avetisova G.Ye., Hambardzumyan A.A., Chakhalyan A.Kh., Saghyan A.S. Study of Regulation of Alanine Transaminase in the Brevibacterium flavum Strain-Producers. Abstract in book of articles and abstracts of 2nd International Scientific Conference of Young Researchers on Biotechnology, General and Applied Microbiology, Chemistry, Biochemistry, Molecular Biology and Genetics, Environmental Protection “Contribution of young generation in the development of biotechnology”, Dedicated to the 70th Anniversary of the National Academy of Sciences, October 1-4, 2013, Yerevan, Armenia, p. 138.
  12. Dyukova K.G., Izmailyan M.S., Ghochikyan V.T.. Isolation and Purification of Inulinase from Jerusalem Artichoke Halanthus tuberosus Tubers. “Contribution of the young generation in the development of biotechnology” 2 nd International Scientific Conference of Young Reserchers. October 1-4, 2013, Yerevan, p. 160-161.
  13. Hambardzumyan A. Mkhitaryan A., Paloyan A. Immobilization of new D-aminoacylase of AM 6.1 strain. Thesis in Book of Abstracts of International Scientific Workshop “Trends in Microbiology and Microbial Biotechnology”, October 5-8, Yerevan, Armenia, 2014, p. 57.
  14. Gyulkhandanyan A.G., Tuchina E.S., Ghazaryan R.K., Paronyan M.H., Dzhagarov B.M., Gyulkhandanyan G.V., Tuchin V.V. Photophysical properties of porphyrins as a selection criterion of porphyrins for study of their effectiveness against microorganisms. FEBS Journal, 2014; 281(suppl.1):665.
  15. Aganyants H., Hovsepyan A., Hovhannisyan Ye., Sakanyan V. Production of recombinant d-hydantoinase of thermophilic bacteria Geobacillus stearothermophilus. Book of abstracts of International Scientific Workshop “Trends in microbiology and microbial biothechnology”, Yerevan, Armenia, October 5–8, 2014, p.41. ISBN 978-5-8084-1895-0.
  16. Aghajanyan A., Hovsepyan A., Hovhannisyan G., Yeghiyan K., Avetisyan S. Isolation of water-soluble melanin from fermentation broth and it’s characterization. Book of Abstracts of International Scientific Workshop “Trends in microbiology and microbial biotechnology”, Yerevan, Armenia, October 5–8, 2014, p. 42. ISBN 978-5-8084-1895-0.
  17. Gyulkhandanyan A.G., Tuchina E.S., Ghazaryan R.K., Paronyan M.H., Dzhagarov B.M., Gyulkhandanyan G.V., Tuchin V.V. Quantum yield of singlet oxygen generation by porphyrins and their efficacy against microorganisms. Book of Abstracts of International Scientific Workshop “Trends in Microbiology and Microbial Biotechnology”, Yerevan, Armenia, 5–8 October, 2014, p. 56. ISBN 978-5-8084-1895-0.
  18. Hovsepyan A., Avetisyan S., Aghajanyan A., Azaryan K., Petrosyan M., Mikayelyan N., Popov Yu. Perspectives of application of biologically active water-soluble melanin of Bacillus thuringiensis in agriculture. Book of Abstracts of International Scientific Workshop “Trends in microbiology and microbial biotechnology”, Yerevan, Armenia, October 5–8, 2014, p. 64. ISBN 978-5-8084-1895-0.
  19. Hovsepyan A., Koloyan H., Hovhannisyan Ye. Moleucular cloning of argB and argJ genes of thermophilic bacterium Bacillus stearothermophilus in the cells of Brevibacterium flavum. Book of abstracts of International Scientific Workshop “Trends in microbiology and microbial biothechnology”, Yerevan, Armenia, October 5–8, 2014, p.65. ISBN 978-5-8084-1895-0.
  20. Paronyan M.H., Gyulkhandanyan G.V., Ghazaryan R.K. Bactericidal effect of photodynamic inactivation against methicillin-resistant and methicillin-susceptible Staphylococcus aureus. Book of abstracts of International Scientific Workshop “Trends in Microbiology and Microbial Biotechnology”, Yerevan, Armenia, 5–8 October, 2014, p. 80. ISBN 978-5-8084-1895-0.
  21. Petrosyan M., Avetisova G., Hovsepyan A., Sahakyan A., Azaryan K., Sahakyan N. Investigation of mutual influence of bacterial bio-preparations “AZOTOVIT-1” and “MELANIN” on the yield of Solánum tuberósum. Book of abstracts of International Scientific Workshop “Trends in microbiology and microbial biothechnology”, Yerevan, Armenia, October 5–8, 2014, p.81. ISBN 978-5-8084-1895-0.
  22. Paloyan A., Mkhitaryan A., Hambardzumyan A. Substrate specificity of new D-aminoacylase of AM 6.1 strain. Thesis in Book of Abstracts of International Scientific Workshop “Trends in Microbiology and Microbial Biotechnology”, October 5-8, Yerevan, Armenia, 2014, p. 79.
  23. Paloyan A.M., Mkhitaryan A.V., Hambardzumyan A.A., Saghyan A.S. Characterization and application of AM6.1 strain belonging to Alcaligenaceae family. Conferece report and proceedings, Royal Socity of Chemistry Translational Biocatalysis, 16th December 2014, RSC Burligton House, Piccadilly, London, UK, p. 11.
  24. Yepremyan H.S. The technology of producing of aminoacylase biocatalyst development. Abst. of reports on 8-th Moscow International Congress “Biotechnology state of the art and prospects of development”, 2015, Moscow, March 17-20, p. 384-385.
  25. Avetisyan S.V., Fomenko T.I., Aghajanyan A.E., Azaryan K.G., Tadevosyan P.Ye., Mikayelyan N.E., Hovsepyan A.S. Effect of various metal ions and vitamins on the biosynthesis of melanin by Bacillus thuringiensis K1 strain. Book of Abstracts of III International Scientific Conference of Young Researchers ״Dialogue on Sciences״, Yerevan, Armenia, June 23–26, 2015, p. 9. ISBN 978-9939-1-0196-5.
  26. Aganyants H.A., Hovhannisyan Ye.A. Hovsepyan A.S., Lecocq M., Sakanyan V.A. Site-directed mutagenesis of recombinant D-hydantoinase of thermophilic bacteria Geobacillus Stearothermophilus. Book of Abstracts of III International Scientific Conference of Young Researchers ״Dialogue on Sciences״, Yerevan, Armenia, June 23–26, 2015, p.50-51. ISBN 978-9939-1-0196-5.
  27. Hovsepyan A.S., Koloyan H. O. Construction of highly active recombinant strain producer of L-arginine on the basis of Brevibacterium flavum containing argB gene of heterologous origin. Book of Abstracts of III International Scientific Conference of Young Researchers “Dialogue on Sciences”, Yerevan, Armenia, June 23–26, 2015, p. 52. ISBN 978-9939-1-0196-5.
  28. Paloyan A.M., Mkhitaryan A.V. and Hambardzumyan A.A. Production of L-phenylalanine catalyzed by coupled enzymatic system of aminotransferase and aspartase of Erwinia carotovora. Thesis in Book of Abstracts of 3rd International Scientific Conference on “Dialogues on Scienses” June 23-26, 2015, Yerevan, Armenia, ISBN 978-9939-1-0196-5, p. 67.
  29. Dyukova K.G., Izmailyan M.S., Ghochikyan V.T. Purification and characterization of inulinase from Jerusalem Artichoke (Helianthus tuberosus) tubers. 3rd International Scientific Conference on “Dialogues on sciences” June 23-26, 2015, Yerevan, Armenia, p 68.
  30. Hambardzumyan A.A., Koloyan H.O., Paloyan A.M., Mkhitaryan A.V., Melkonyan L.H. Cloning of genes of -amylase and glucoamylase of technological value and characterization of corresponding enzymes Thesis in Book of Abstracts of 3rd International Scientific Conference on “Dialogues on Scienses” June 23-26, 2015, Yerevan, Armenia, ISBN 978-9939-1-0196-5, p. 71.
  31. Paloyan A., Melkonyan L., Mkhitaryan A., Hambardzumyan A., Saghyan A. Purification and characterization of α-amylase from Bacillus amyloliquifaciens. Thesis in Book of Abstracts of 7nd Congress of Europian microbiologists, July 9-13, 2017, Valencia, Spain, p.240.
  32. Paronyan M., Hovsepyan A., Ghazaryan R. and Gyulkhandanyan G. Study of the possibility of resistance of gram-positive and gram-negative bacteria to photodynamic inactivation when using metalloporphyrin Zn-TBut4PyP. Book of Abstracts of the 4th International Scientific Conference of Young Researchers “Biotechnology: Science and Practice”, Yerevan, Armenia, September 28–30, 2017, p. 10. ISBN 978-9939-1-0620-5.
  33. Paloyan A., Melkonyan L., Sargsyan A., Mkhitaryan A., Hambardzumyan A. Substrate specificity of  -amylase isolated from Bacillus amyloliquefacience strain MDC 1974. Thesis in Book of Abstracts of 4th International Scientific Conferens of Young Researchers “Biotechnology: Science and Practice”, ISBN 978-9939-1-0620-5, Yerevan, Scientific and Production Center «Armbiotechnology» NAS RA, September 28-30, 2017, p.37-38.
  34. Hovsepyan A., Avetisyan S., Tadevosyan P., Koloyan H., Filipenia V. and Chizhik O. Obtaining of new highly effective insecticidal strains based on melaninogenic mutants of Bacillus thuringiensis. Book of Abstracts of 4th International Scientific Conference of Young Researchers “Biotechnology: Science and Practice”, Yerevan, Armenia, September 28–30, 2017, p. 46–47. ISBN 978-9939-1-0620-5.
  35. Dyukova K.G., Izmailyan M.S. and Ghochikyan V.T. Inactivation of fumarase of P.carotavorum with aspartic activity. “Biotechnology: science and practice” 4th International Scientific Conference of Young Researchers. September 28-30, 2017, Yerevan, Armenia, p.56-57.
  36. Yepremyan H. S. Artsruni G. K. Research of substrate specifity of intracellular aminoacylase of Escherichia coli strain LGE 36. Abstr. of reports on 4th International Scientific conference of Young Researchers. “Biotechnology. Science and Practice, 2017, Yerevan, RA, September 28-30, 64-65.
  37. Koloyan H., Hovsepyan A. and Hovhannisyan Y. Ways of increasing arginine-producing ability of recombinant strain Brevibacterium flavum. Book of Abstracts of 4th International Scientific Conference of Young Researchers “Biotechnology: Science and Practice”, Yerevan, Armenia, September 28–30, 2017, p. 84. ISBN 978-9939-1-0620-5
  38. Aganyants H., Hovhannisyan E., Vardanyan T., Hovsepyan A., Hambardzumyan A. and Sakanyan V.  In silico analysis and biochemical study of mutant D-hydantoinases of Geobacillus stearothermophilus descent. Thesis in Book of Abstracts of 4th International Scientific Conferens of Young Researchers “Biotechnology: Science and Practice”, ISBN 978-9939-1-0620-5, Yerevan, Scientific and Production Center «Armbiotechnology» NAS RA, September 28-30, 2017, p.89-90.
  39. Hambardzumyan A.  Statistical analysis of enzyme kinetics: simple Michaelis-Menten and bi-bi ping-pong; inhibition. Thesis in Book of Abstracts of 4th International Scientific Conferens of Young Researchers “Biotechnology: Science and Practice”, ISBN 978-9939-1-0620-5, Yerevan, Scientific and Production Center «Armbiotechnology» NAS RA, September 28-30, 2017, p.99-100.
  40. Grigoryan A.M., Hambardzumyan A.A., Mikaelyan A.R., Melkonyan M.M. Comparative docking of Mesedine and Beditine with homology model of alpha2a-adrenergic receptor. “Science Week” Conference Abstracts, November 27th – December 1st in: The New Armenian Medical Journal, Vol.11, 2017, No3, P.21, Supplement.
  41. Paloyan A., Melkonyan L., Sargsyan A., Arakelyan T., Izmailyan M., Avetisova G., Hambardzumyan A., Sagyan A. Optimization of fermentation condition for -amylase production; Abstracts submitted to the 43rd FEBS Congress, taking place in Prague, Czech Republic from 7th to 12th July 2018, Poster P.07-038-Tue, p. 177, FEBS Open Bio 8 (Suppl. S1) (2018) 107–496 DOI: 10.1002/2211-5463.12453 177 Є 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
  42. Melkonyan L., Paloyan A., Hambardzumyan A., Avetisova G., Mkhitaryan A., Sargsyan A., Stepanyan S., Arabachyan S., Saghyan A. Study of some metals effect on -amylase activity of Bacillus amyloliquefaciens MDC 1974 strain; Abstracts submitted to the 43rd FEBS Congress, taking place in Prague, Czech Republic from 7th to 12th July 2018, Poster P.07-042-Wed, p. 178,  FEBS Open Bio 8 (Suppl. S1) (2018) 107–496 DOI: 10.1002/2211-5463.12453 177 Є 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
  43. Grigoryan A., Hambardzumyan A., Mikaelyan A. and Melkonyan M. A Comparative Study of Potential Ligands to the Homology Model of Human Alpha2 Adrenoceptor, Abstracts submitted to the 4th Neurological Disorders Summit, Featured Presentation, July 23-25, 2018, Venue Four Points by Sheraton Los Angeles International Airport 9750 Airport Boulevard, Los Angeles, CA 90045, United States, p. 17-18.
  44. Grigoryan A., Babayan B., Melkonyan M., Hambardzumyan A. and Mikaelyan A. Identification of Binding Affinities of Some Pyrethroid-Like Cyclopropane/Cyclobutane Ligands with VG SCN and NMDA Receptor, Abstracts submitted to the 4th Neurological Disorders Summit, Poster Presentation, July 23-25, 2018, Venue Four Points by Sheraton Los Angeles International Airport 9750 Airport Boulevard, Los Angeles, CA 90045, United States, p. 75-76.
  45. Grigoryan A., Babayan B., Avetisyan S., Sargsyan V., Hambardzumyan A., Mikaelyan A. Comparative docking analysis of Cyclopropane/Cyclobutane derivatives with human modeled and bacterial voltage gated sodium channels and NMDA receptor, Thesis in Book of Abstracts of 1st International Scientific Conference “Current State of Pharmacy and Prospects of its Development”, Report, November 01-03, 2018, Yerevan, Armenia, p. 30-31.
  46. Aganyanc H., Hovhannisyan Y., Toplaghaltsyan A., Hambardzumyan A., Hovsepyan A., Sakanyan V. Approaches for obtaining new highly active recombinant thermostable D-hydantoinases, Thesis in Book of Abstracts of 1st International Scientific Conference “Current State of Pharmacy and Prospects of its Development”, Poster Presentation, November 01-03, 2018, Yerevan, Armenia, p. 89.
  47. Paloyan A., Melkonyan L., Hambardzumyan A., Mkhitaryan A., Avetisova G. New -amylase from Bacillus sp. MDC 3500, Poster presentation, Thesis in Book of Abstracts of International Conference “Designer Biology: From proteins and cells to scaffolds & materials in Europe”, Newcastle University, UK, 31st July – 2nd August 2019, P. 47.
  48. Hambardzumyan A.A. Application of aminotransferases in asymmetric syntheses of amino acids, Plenary Lecture, Thesis in Book of Abstracts of International Conference “Microbes: Biology&Application”, October 9-11, 2019, Yerevan, Armenia, ISBN 978-9939-1-1010-3, P. 27-28.
  49. Ghazaryan V.G., Mkhitaryan A.V., Paloyan A.A., Hambardzumyan A.A., Saghyan A.S. Possibility of using thermophilic L-aminoacylases of bacilli origin in the asymmetric syntheses of amino acids, Thesis in Book of Abstracts of International Conference “Microbes: Biology&Application”, October 9-11, 2019, Yerevan, Armenia, ISBN 978-9939-1-1010-3, P. 56-579.
  50. Mkhitaryan A.V., Paloyan A.A., Ghazaryan V.G., Hambardzumyan A.A. New Aminoacylase strain from Rhodococcus genus, Thesis in Book of Abstracts of International Conference “Microbes: Biology&Application”, October 9-11, 2019, Yerevan, Armenia, ISBN 978-9939-1-1010-3, P. 58-59.
  51. Paloyan A., Melkonyan L., Mkhitaryan A., Dyukova K., Hambardzumyan A. New -amylase with the potential for commercialization, Poster presentation, Thesis in Book of Abstracts of International Conference “Microbes: Biology&Application”, October 9-11, 2019, Yerevan, Armenia, ISBN 978-9939-1-1010-3, P. 60.
  52. Dyukova K.G., Izmailyan M.S., Hambardzumyan A.A. Biosynthesis of extracellular glucose oxidase by Penicillium chrysogenum, Thesis in Book of Abstracts of International Conference “Microbes: Biology&Application”, October 9-11, 2019, Yerevan, Armenia, ISBN 978-9939-1-1010-3, P. 54-55.
  53. Yepremyan H.S. Approaches to studying the activity and stability of intracellular recombinant aminoacylase of Escherichia coli, Thesis in Book of Abstracts of International Conference “Microbes: Biology&Application”, October 9-11, 2019, Yerevan, Armenia, ISBN 978-9939-1-1010-3, P. 14.
  54. Paronyan M., Avetisyan S., Koloyan H., Hovsepyan A., Gyulkhandanyan  G. Photodynamic inactivation of methicillin-resistant Staphylococcus aureus and methicillin-sensitive Staphylococcus aureus. International Conference “Microbes: Biology & Application October 9-11, 2019, Yerevan, Armenia. Book of Abstracts, p. 24. ISBN 978-9939-1-1010-3
  55. Avetisyan S.V., Tadevosyan P.Ye., Koloyan H.O., Paronyan M.H., Hovsepyan A.S. Insecticidal preparations and plant growth stimulator based on melaninogenic Bacillus thuringiensis strains. International Conference “Microbes: Biology & Application October 9-11, 2019, Yerevan, Armenia. Book of Abstracts, p. 42. ISBN 978-9939-1-1010-3
  56. Koloyan H.O., Avetisyan S.V., Paronyan M.H., Aganyants H.A., Hovsepyan   A.S. Creation of new recombinant strain-producers of l-arginine using shuttle expression vector pEC-XK99E. International Conference “Microbes: Biology & Application October 9-11, 2019, Yerevan, Armenia. Book of Abstracts, p. 49. ISBN 978-9939-1-1010-3
  57. Aganyants H., Toplaghaltsyan A., Hovhannisyan Ye., Hovsepyan A., Sakanyan V. Influence of phe65ala point mutation on substrate specificity of Geobacillus stearothermophilus d-hydantoinase. International Conference “Microbes: Biology & Application October 9-11, 2019, Yerevan, Armenia. Book of Abstracts, p. 50. ISBN 978-9939-1-1010-3

Staff

Anichka

Hovsepyan

Leading Researcher,

PhD in Biology

anichkahovsepyan@armbiotech.am  

Hasmik

Yepremyan

Senior Researcher,

PhD in Chemistry

hasmikepremyan54@mail.ru 

Haykanush

Koloyan

Senior Researcher,

PhD in Biology

ankoloyan@armbiotech.am 

Karine

Dyukova

Senior Researcher,

PhD in Chemistry

karinedyukova@armbiotech.am 

Ani

Paloyan

Senior Researcher,

PhD in Biology

 anipaloyan@armbiotech.am

Sona

Avetisyan

Senior Researcher,

PhD in biology

 sonaavetisyan@armbiotech.am

Marina

Paronyan

Researcher,

PhD in Biology

 marinaparonyan@armbiotech.am 

Anna

Mkhitaryan

Junior Researcher,

PhD in Biology

 annamkhitaryan@armbiotech.am

Marieta

Izmailyan

 

Junior Researcher

 

Narine

Mikayelyan

 

Laboratory Assistant